Modification of Bovine β-Lactoglobulin by Glycation in a Powdered State or in an Aqueous Solution:  Effect on Association Behavior and Protein Conformation - l'unam - université nantes angers le mans Accéder directement au contenu
Article Dans Une Revue Journal of Agricultural and Food Chemistry Année : 1999

Modification of Bovine β-Lactoglobulin by Glycation in a Powdered State or in an Aqueous Solution:  Effect on Association Behavior and Protein Conformation

Résumé

The effect of glycation with lactose on the association behavior and conformational state of bovine β-lactoglobulin (β-LG) was studied, using size exclusion chromatography, polyacrylamide gel electrophoresis, proteolytic susceptibility, and binding of a fluorescent probe. Two modification treatments were used, i.e., aqueous solution glycation and dry-way glycation. The results showed that the latter treatment did not significantly alter the nativelike behavior of the protein while the former treatment led to important structural changes. These changes resulted in a specific denatured β-LG monomer, which covalently associated via the free thiol group. The homodimers thus formed and the expanded monomers underwent subsequent aggregation into a high molecular weight species, via noncovalent interactions. The association behavior of glycated β-LG is discussed with respect to the known multistep denaturation/aggregation process of nonmodified β-LG.
Fichier principal
Vignette du fichier
jf9804387_{866AE07B-F797-40E2-BD48-E51DB69EFC4F}.pdf (138.22 Ko) Télécharger le fichier
Origine : Accord explicite pour ce dépôt
Loading...

Dates et versions

hal-01569909 , version 1 (27-07-2017)

Licence

Paternité - Partage selon les Conditions Initiales

Identifiants

Citer

François Morgan, Joelle Léonil, Daniel Mollé, Said Bouhallab. Modification of Bovine β-Lactoglobulin by Glycation in a Powdered State or in an Aqueous Solution:  Effect on Association Behavior and Protein Conformation. Journal of Agricultural and Food Chemistry, 1999, 47 (1), pp.83-91. ⟨10.1021/jf9804387⟩. ⟨hal-01569909⟩
54 Consultations
132 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More